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List of alignment visualization software

This page is a subsection of List of sequence alignment software.

Multiple alignment visualization tools typically serve four purposes:

  • General comprehension of large-scale DNA or protein alignments
  • Visualization of alignments for figures and publication.
  • Manual editing and curation of automatically generated alignments.
  • In depth analysis

The rest of this article is focused on just multiple global alignments of homologous proteins. The first two are a natural consequence of the fact that most computational representations of alignments and their annotation are not human readable and best portrayed in the familiar sequence row and alignment column format, of which examples are widespread in the literature. The third is a necessity because both Multiple sequence alignment and Structural alignment algorithms utilise heuristics which do not always perform perfectly. The fourth is a great example of how interactive graphical tools enable a worker involved in sequence analysis to conveniently execute a variety if different computational tools in order to explore an alignment's phylogenetic implications; or, to predict the structure and functional properties of a specific sequence (e.g. comparative modelling).

Alignment viewers/editors

NameIntegrated with Struct. Prediction ToolsCan Align SequencesCan Calculate Phylogenetic TreesOther FeaturesFormats SupportedLicenseLink
Ale (emacs plugin)NoYesNoNoGenBank, EMBL, Fasta, and PhylipGPLlink
Base-By-BaseNoMuscleUPGMA, NJ, Complete Linkage, Single Linkage, WPMGAVisual summary, percent identity tables, some integrated advanced analysis toolsGenbank, Fasta, EMBEL, Clustal, Base-by-base filesFree, registration requiredlink
BioEditNoClustalWrudimentary, can read phylipplasmid drawing, ABI chromatograms,Genbank, Fasta, Phylip 3.2, Phylip 4, NBRF/PIRFreelink
BioNumericsNoYesYes Genbank, FastaCommerciallink
BoxShadeNoNoNoSpecifically for multiple alignmentsMSF format as written by PILEUP, READSEQ, or SEQIO (fmtseq). ALN format as written by CLUSTALW.Public domainlink
CINEMANO, but can read/show 2D structure annotationsClustalWNoDotplot, 6 frame translation, BlastNexus, MSF, Clustal, FASTA, PHYLIP, PIR, PRINTSFreelink
CLC viewer (Free version)only in commercial versionClustal, Muscle, T-Coffee, MAFFT, kalign, variousUPGMA, NJworkflows, blast/genbank searchmanyFreeware. More options available in commercial versions.link, table of features
ClustalX viewerNoClustalwNeighbor-joiningAlignment quality analysisNexus, MSF, Clustal, FASTA, PHYLIPFree for academic userslink
Cylindrical BLAST ViewerNoNoNo3D, Animation, Drilldown, Legend SelectionBLAST XML, proprietary XML, GFF3, ClustalW, INSDSet, user expandable with XSLTGPLlink
DnaSP   can compute several population genetics statistics, reconstruct haplotypes with PHASEFASTA, Nexus, Mega, PHYLIPFreewarelink
emacs - biomode      link
GenedocNo, but can read/show annotationsPairwiseNo, but can read/show annotationsgel simulation, stats, multiple views, simplemanyFreelink table of features
Geneious ProYes - powered by EMBOSS toolsClustal, Muscle, MAUVE, profile, translationUPGMA, NJ, PhyML, MrBayes plugin, PAUP* pluginWhole genome assembly, restriction analysis, cloning, primer design, dotplot and much more>40 file formats imported and exportedGeneious Basic (freeware) Geneious Pro (commercial with student and academic discounts)link
Integrated Genome Browser (IGB)NoNoNosequences and features from files, URLs, and arbitrary DAS and QuickLoad serversBAM, FASTA, PSLCPLlink
Jalview 2Secondary Struct. Prediction via JNETClustal, Muscle, MAFFT, Probcons, TCoffee via web servicesUPGMA, NJsequences and features from arbitrary and publicly registered DAS servers, PFAM, PDB, EMBL and Uniprot Accession retrieval.FASTA, PFAM, MSF, Clustal, BLC, PIR, StockholmGPLlink
JEvTraceNo but integrated with structure viewer WebMolNoNoA multivalent browser for sequence alignment, phylogeny, and structure. Performs an interactive Evolutionary Trace and other phylogeny inspired analysis.FASTA, MSF, Clustal, PHYLIP, Newick, PDBFree for academic uselink manual
MEGANoNative ClustalWUPGMA, NJ, ME, MP, with bootstrap and confidence testextended support to phylogenetics analysisFASTA, Clustal, Nexus, Mega, etc..Freeware, registration requestedlink, table of features
Multiseq (vmd plugin)No, but can display and align 3D structuresClustaLW, MaFFT, Stamp (Structural)Percent identity, Clustal, MaFFT, StructuralScripting via Tcl, mapping from sequence to 3D structureFASTA, PDB, ALN, PHYLYP, NEXUSFree, but VMD is free only for noncommercial uselink
MViewNoNoNostacked alignments from blast and fasta suites, various MSA format conversions, HTML markup, consensus patternsBLAST search, FASTA search, Clustal, HSSP, FASTA, PIR, MSFGPLlink
PFAATNO, but can display 3D structuresClustalWNeighbor-joiningManual annotation, conservation scoresNexus, MSF, Clustal, FASTA, PFAATFreelink
Ralee (emacs plugin for RNA al. editing) RNA structure  StockholmGPLlink
S2S RNA editor2D structureRnalignNobase-base interactions,2D,3D viewerFASTA, RnaMLFreelink
SeaviewNolocal Muscle/ClustalwParcimony, distance methods, PhyMLDot-plot, vim-like editing keysNexus, MSF, Clustal, FASTA, PHYLIP, MASE link
SequilabYesYesNoLink alignment results to analysis tools (Primer design, Gel mobility and Maps, Plasmapper, siRNA design Epitope prediction), Save research logs, Create custom toolbarsAccession number, GI number, PDB ID, FASTA, DragNdrop from external URL from within the user interfaceFreewarelink
SeqPopNo    Freelink
StrapJnet, NNPREDICT, Coiled coil, 16 different TM-helixFifteen different MethodsNeighbor-joiningDot-plot, Structure-neighbors, 3D-superposition, Blast-search, Mutation/SNP analysis, Sequence features, Biojava-interfaceMSF, Stockholm, Clustalw, Nexus, FASTA, PDB, Embl, GenBank, hssp, PfamGPLlink
UGENEYesMUSCLE, KAlignYesmanyFASTA, FASTQ, GenBank, EMBL, ABIF, SCF, CLUSTALW, Stockholm, Newick, PDB, MSF, GFFGPLlink
VISSA sequence/structure viewerDSSP secondary structureClustalXNoMapping from sequence to 3D structureClustal, FASTAFreelink
MaestroYesClustalXYesMapping from sequence to 3D structure, structure/sequence editing/modelingClustal, FASTA PDBFree for Academicslink

Some useful discussions on sequence alignment editors/viewers can be found here:

See also

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